Saccharification of cassava starch using Aspergillus niger ITB CC L74

Ukan Sukandar, Achmad Ali Syamsuriputra, Lindawati L, Yadi Trusmiyadi

Abstract


Bioethanol is a derivative product from cassava that is thoroughly developed nowadays. One of the bioethanol research program of the Chemical Engineering Product Design and Development Research Group, Faculty of Industrial Technology, Institut Teknologi Bandung in the year of 2008 was to increase productivity and the performance of a-amylase and glucoamylase from Aspergillus niger ITB CC L74 for saccharification of cassava starch in bioethanol production. In conjunction with the optimum condition of saccharification process, research on the effect of pH, temperature, Ca2+ concentration, substrate concentration, and enzyme volume percentage have been carried out. The performance of a-amylase was determined by the iodine method while the performance of glucoamylase was done by the Somogyi-Nelson method. Research variable to determine the optimum performance condition of amylase extract were pH (3.15–7.0), temperature (30–89 oC), Ca2+ concentration (0–200 ppm), substrate concentration (0.5–20 %-w/v), and enzyme volume percentage (1.0–50 %-v). The results of this research showed that the optimum performance of amylase complex were pH 4.5, temperature 60 oC, Ca2+ concentration 75 m/L, and substrate concentration 7 %-w/v. The performances of amylase complex increased with the increase in the amount of enzyme percentage, but the increase was limited by the amount of glucose that could inhibit enzyme activity.

Keywords


amylase; glucoamylase; Aspergillus niger; saccharification; starch

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